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KMID : 1094720000050020123
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 2 p.123 ~ p.129
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Development of high density mammalian cell culture system for the production of tissue-type plasminogen activator
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Park Byong-Gon
Chun Joo-Mi
Lee Chang-Jin
Chun Gie-Taek
Kim Ik-Hwan
Jeong Yeon-Ho
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Abstract
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A high cell density culture system for the anchorage dependent CHO cells was developed based on the combination ofin situ removal of ammonium ion and microcarrier culture system, and semi-fed-batch feeding of glucose and glutamine was employed to the developed culture system. The glass bead was selected as an optimum microcarrier in terms of cell growth. An ammonium ion selective zeolite, Phillipsite-Gismondine, was packed in a dialysis membrane and equipped on the agitator of spinner reactor forin situ removal of ammonium ion. The semi-fed-batch operation was employed to the novel culture system for the high density cell culture, and the results showed the cell growth was improved by 32% and tPA productivity by 250%.
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KEYWORD
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CHO cells, tPA, zeolite, in situ ammonium ion removal, microcarrier, semi-fedbatch
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